ON A SIMPLIFIED METHOD OF STAINING ACID-FAST BACTERIA. II.
نویسندگان
چکیده
منابع مشابه
Safer staining method for acid fast bacilli.
To develop a method for staining acid fast bacilli which excluded highly toxic phenol from the staining solution. A lipophilic agent, a liquid organic detergent, LOC High Studs, distributed by Amway, was substituted. The acid fast bacilli stained red; nuclei, cytoplasm, and cytoplasmic elements stained blue on a clear background. These results compare very favourably with acid fast bacilli stai...
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A reliable, convenient, and accurate method for counting ac id-fast hacteria ( AFB) is essential for much work with Mycobacterium leprae. The procedure used , previously in the laboratory (~, 4 ) had two technical difficulties: (a) In order for the drops to spread satisfactorily the microscope slides had to have a uniform wetability of the correct degree; sometimes this requirement was difficul...
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A dual-staining procedure for acid-fast bacilli was found to have poor correlation with the Ziehl-Neelsen and auramine-rhodamine staining techniques.
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In a previously reported attempt (Gordon, 1937) to find standards for the classification of the rapidly-growing, non-pathogenic mycobacteria, we presented the results of a cultural study of a collection of 252 strains from soil, plants, human and animal tissues and secretions. Eighty per cent of the strains were readily separated into three groups of closely-related cultures. The remaining 20 p...
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The possession of mucinous envelopes by certain bacteria, apart from their purely morphological interest, has a great value in the identification of such organisms and many methods have been devised for their demonstration. The number of such methods however argues against any one being completely satisfactory. The introduction of a new technique into this already overcrowded field can be justi...
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ژورنال
عنوان ژورنال: The Japanese Journal of Veterinary Science
سال: 1940
ISSN: 0021-5295,1881-1442
DOI: 10.1292/jvms1939.2.488